Article
Hepatitis C genotyping by direct sequencing of the product from the Roche AMPLICOR test: methodology and application to a South Australian population.
Pathology - 1 May 1998
Holland J, Bastian I, Ratcliff R M, Beers M Y, Hahesy P, Harley H, Shaw D R, Higgins G D
Abstract excerpt
The Roche AMPLICOR RT-PCR amplifies a 244 nucleotide sequence within the 5' non coding region (5'NCR) of the viral genome and is a widely used commercial test for the qualitative determination of hepatitis C RNA from sera. This paper describes a routine procedure for the purification of the PCR product, and its use in automated DNA sequencing, for determining the genotype of hepatitis C virus (HCV) isolates....
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.