Hepatitis C genotyping by direct sequencing of the product from the Roche AMPLICOR test: methodology and application to a South Australian population.

doi:10.1080/00313029800169226

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Article

Hepatitis C genotyping by direct sequencing of the product from the Roche AMPLICOR test: methodology and application to a South Australian population.

Pathology - 1 May 1998

Holland J, Bastian I, Ratcliff R M, Beers M Y, Hahesy P, Harley H, Shaw D R, Higgins G D

Abstract excerpt

The Roche AMPLICOR RT-PCR amplifies a 244 nucleotide sequence within the 5' non coding region (5'NCR) of the viral genome and is a widely used commercial test for the qualitative determination of hepatitis C RNA from sera. This paper describes a routine procedure for the purification of the PCR p...

Topics

Adult
Australia
DNA, Viral
Female
Genotype
Hepacivirus
Humans
Male
Middle Aged
Molecular Biology
Polymerase Chain Reaction
RNA, Viral
Transcription, Genetic

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