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Article

Determination of hepatitis C virus genotypes in the United States by cleavase fragment length polymorphism analysis.

Journal of clinical microbiology - 1 Dec 1997

Marshall D J, Heisler L M, Lyamichev V, Murvine C, Olive D M, Ehrlich G D, Neri B P, de Arruda M

Abstract excerpt

We describe the application of a new DNA-scanning method, which has been termed Cleavase Fragment Length Polymorphism (CFLP; Third Wave Technologies, Inc., Madison, Wis.), for the determination of the genotype of hepatitis C virus (HCV). CFLP analysis results in the generation of structural fingerprints that allow discrimination of different DNA sequences. We analyzed 251-bp cDNA products generated by reverse...

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Identifiers

PMID: 9399512
DOI: 10.1128/jcm.35.12.3156-3162.1997
PMCID: PMC230140

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