Article
Characterization of a SV40-transformed rheumatoid synovial fibroblast cell line which retains genotypic expression patterns: a model for evaluation of anti-arthritic agents.
In vitro cellular & developmental biology. Animal - 1 Jan 1997
Zhang H G, Blackburn W D, Minghetti P P
Abstract excerpt
A chimeric Adenovirus-Simian Virus 40 (AdSV40) containing the large T antigen was used to transform rheumatoid synovial fibroblasts. A rheumatoid synovial fibroblast cell line was established by infection of primary rheumatoid arthritis (RA) synovial fibroblasts at Passage 10 with AdSV40 recombin...
Topics
- Antirheumatic Agents
- Arthritis, Rheumatoid
- Cell Line, Transformed
- Dexamethasone
- Fibroblasts
- Fluorescent Antibody Technique
- Gene Expression Regulation, Enzymologic
- Genes, jun
- Genotype
- Humans
- Interleukin-1
- Metalloendopeptidases
- Models, Biological
- Simian virus 40
- Synovial Membrane
- Tetradecanoylphorbol Acetate
- Transforming Growth Factor alpha