Article
Interleukin-1 beta-modulated gene expression in immortalized human chondrocytes.
The Journal of clinical investigation - 1 Dec 1994
Goldring M B, Birkhead J R, Suen L F, Yamin R, Mizuno S, Glowacki J, Arbiser J L, Apperley J F
Abstract excerpt
Immortalized human chondrocytes were established by transfection of primary cultures of juvenile costal chondrocytes with vectors encoding simian virus 40 large T antigen and selection in suspension culture over agarose. Stable cell lines were generated that exhibited chondrocyte morphology, cont...
Topics
- Antigens, Viral, Tumor
- Cartilage
- Cell Line
- Cell Transformation, Viral
- Chondroitin Sulfates
- Collagen
- Cycloheximide
- Fluorescent Antibody Technique
- Gene Expression
- Humans
- Immunohistochemistry
- Interleukin-1
- Phenotype
- Proteoglycans
- RNA, Messenger
- Ribs
- Simian virus 40