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Article

A novel positive selection for identifying cold-sensitive myosin II mutants in Dictyostelium.

Genetics - 1 Jun 1995

Patterson B, Spudich J A

Abstract excerpt

We developed a positive selection for myosin heavy chain mutants in Dictyostelium. This selection is based on the fact that brief exposure to azide causes wild-type cells to release from the substrate, whereas myosin null cells remain adherent. This procedure assays myosin function on a time scale of minutes and has therefore allowed us to select rapid-onset cold-sensitive mutants after random chemical...

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Identifiers

PMID: 7498732
DOI: 10.1093/genetics/140.2.505
PMCID: PMC1206630

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