Article
A novel positive selection for identifying cold-sensitive myosin II mutants in Dictyostelium.
Genetics - 1 Jun 1995
Patterson B, Spudich J A
Abstract excerpt
We developed a positive selection for myosin heavy chain mutants in Dictyostelium. This selection is based on the fact that brief exposure to azide causes wild-type cells to release from the substrate, whereas myosin null cells remain adherent. This procedure assays myosin function on a time scal...
Topics
- Animals
- Azides
- Base Sequence
- Chromosome Mapping
- Cold Temperature
- Dictyostelium
- Genetic Markers
- Indicators and Reagents
- Molecular Sequence Data
- Mutation
- Myosins
- Oligonucleotide Probes
- Phenotype
- Plasmids
- Selection, Genetic
- Sodium Azide