Article
Transposon mutagenesis of probiotic Lactobacillus casei identifies asnH, an asparagine synthetase gene involved in its immune-activating capacity.
PloS one - 1 Jan 2014
Ito Masahiro, Kim Yun-Gi, Tsuji Hirokazu, Takahashi Takuya, Kiwaki Mayumi, Nomoto Koji, Danbara Hirofumi, Okada Nobuhiko
Abstract excerpt
Lactobacillus casei ATCC 27139 enhances host innate immunity, and the J1 phage-resistant mutants of this strain lose the activity. A transposon insertion mutant library of L. casei ATCC 27139 was constructed, and nine J1 phage-resistant mutants out of them were obtained. Cloning and sequencing analyses identified three independent genes that were disrupted by insertion of the transposon element: asnH, encoding...
Topics
- Amino Acid Sequence
- Animals
- Aspartate-Ammonia Ligase
- Bacterial Proteins
- Bacteriophages
- Cell Wall
- Cytokines
- DNA Transposable Elements
- Disaccharides
- Genes, Bacterial
- Immunity
- Lacticaseibacillus casei
- Listeriosis