Article
Cloning and expression of the Bacteroides fragilis TAL2480 neuraminidase gene, nanH, in Escherichia coli.
Journal of bacteriology - 1 May 1990
Russo T A, Thompson J S, Godoy V G, Malamy M H
Abstract excerpt
We have cloned the Bacteroides fragilis TAL2480 neuraminidase (NANase) structural gene, nanH, in Escherichia coli. This was accomplished by using the cloning shuttle vector pJST61 and a partial Sau3A library of TAL2480 chromosomal inserts created in E. coli. The library was mobilized into the NAN...
Topics
- Amino Acid Sequence
- Bacteroides fragilis
- Base Sequence
- Cloning, Molecular
- DNA Transposable Elements
- DNA, Bacterial
- Escherichia coli
- Gene Expression
- Genes, Bacterial
- Molecular Sequence Data
- Mutation
- Neuraminidase
- Plasmids
- Recombinant Proteins
- Restriction Mapping