Article
Methionine and serine formation in control and mutant human cultured fibroblasts: evidence for methyl trapping and characterization of remethylation defects.
Pediatric research - 1 Jan 1997
Fowler B, Whitehouse C, Wenzel F, Wraith J E
Abstract excerpt
The conversion of labeled formate to methionine and serine, as a measure of remethylation of homocysteine to methionine and folate coenzyme cycling, has been studied in control and mutant human fibroblasts. Fibroblasts in monolayer culture were incubated with [14C]formate, and labeled methionine...
Topics
- 5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase
- Amino Acid Metabolism, Inborn Errors
- Cells, Cultured
- Fibroblasts
- Glycine Hydroxymethyltransferase
- Humans
- Methionine
- Methylenetetrahydrofolate Reductase (NADPH2)
- Mutation
- Oxidoreductases Acting on CH-NH Group Donors
- Proteins
- Serine
- Skin