Article
Identification and perturbation of mutant human fibroblasts based on measurements of methylmalonic acid and total homocysteine in the culture media.
Archives of biochemistry and biophysics - 1 Jun 1993
Kolhouse J F, Stabler S P, Allen R H
Abstract excerpt
Human and mammalian cells contain two cobalamin-dependent enzymes. Methylmalonyl-CoA mutase isomerizes L-methylmalonyl-CoA to succinyl-CoA in the propionyl-CoA pathway while methionine synthase catalyzes the transfer of the methyl group of 5-methyltetrahydrofolate to homocysteine to form methioni...
Topics
- 5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase
- Cell Line
- Culture Media
- Fibroblasts
- Homocysteine
- Humans
- Methylmalonic Acid
- Methylmalonyl-CoA Mutase
- Mutation
- Propionates
- Vitamin B 12