Article
Identification of p53 mutations in archival prostate tumors. Sensitivity of an optimized single-strand conformational polymorphism (SSCP) assay.
Diagnostic molecular pathology : the American journal of surgical pathology, part B - 1 Dec 1996
Shi X B, Bodner S M, deVere White R W, Gumerlock P H
Abstract excerpt
To correlate molecular changes with clinical information in prostate tissue, it is necessary to have accurate methods for screening for mutations in clinically available specimens. We have refined the polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) analysis for detect...
Topics
- DNA Mutational Analysis
- DNA Repair Enzymes
- DNA, Neoplasm
- Exodeoxyribonucleases
- Genes, p53
- Humans
- Male
- Mutation
- Polymerase Chain Reaction
- Polymorphism, Single-Stranded Conformational
- Prostatic Neoplasms
- Tumor Cells, Cultured