Article
Production of monoclonal anti-thymine-dimer antibody and its use for detection of STR alleles.
Nihon hoigaku zasshi = The Japanese journal of legal medicine - 1 Oct 1996
Fukuda M, Tamaki Y, Kishida T, Wang W
Abstract excerpt
To simplify the manual detection of STRs, we developed an immunoblotting method using an anti-thymine-dimer antibody. Upon ultraviolet irradiation, adjacent thymine residues in a DNA strand readily form thymine dimers which are detectable by anti-thymine-dimer antibody. We simply irradiated the supernatant of a boiled suspension of P3U1 myeloma cells with ultraviolet rays, and immunized BALB/c mice with it. By...
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