Article
Functional interaction of transmembrane helices 3 and 6 in rhodopsin. Replacement of phenylalanine 261 by alanine causes reversion of phenotype of a glycine 121 replacement mutant.
The Journal of biological chemistry - 13 Dec 1996
Han M, Lin S W, Minkova M, Smith S O, Sakmar T P
Abstract excerpt
Replacement of a highly conserved glycine residue on transmembrane (TM) helix 3 of bovine rhodopsin (Gly121) by amino acid residues with larger side chains causes a progressive blue-shift in the lambdamax value of the pigment, a decrease in thermal stability, and an increase in reactivity with hydroxylamine. In addition, mutation of Gly121 causes a relative reversal in the selectivity of opsin for 11-cis-retinal...
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