Article
Determinants of visual pigment absorbance: role of charged amino acids in the putative transmembrane segments.
Biochemistry - 30 Jan 1990
Nathans J
Abstract excerpt
I have investigated the effect on bovine rhodopsin's absorbance spectrum of charged amino acid changes in the putative membrane-spanning regions. A total of 14 site-directed mutants were constructed at 6 amino acid positions: 83, 86, 122, 134, 135, and 211. Two of these positions are occupied by charged amino acids that are conserved in all four human visual pigments (positions 134 and 135). In the four variable...
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