Article
A microplate assay for K-ras genotyping.
European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies - 1 Oct 1996
Berndt C, Wolf G, Schröder G, Bebenroth M, Oehlschlegel K, Hillebrand T, Zanow J
Abstract excerpt
The paper presents a microplate hybridisation assay for the detection of codon 12 mutations of the K-ras protooncogene. Single-stranded target DNA, obtained from amplifying sample DNA with 5'-biotin and 5'-digoxigenin-labelled primers and subsequent strand separation, is hybridised with solid phase-fixed capture probes complementary to wild-type and mutated forms of K-ras. After stringent washing the duplex DNA...
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