Article
Genotyping of the human lipoprotein lipase gene by ferrocenylnaphthalene diimide-based electrochemical hybridization assay.
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry - 1 Dec 2005
Nojima Takahiko, Yamashita Kenichi, Takagi Atsuko, Ikeda Yasuyuki, Kondo Hiroki, Takenaka Shigeori
Abstract excerpt
A ferrocenylnaphthalene diimide (FND)-based electrochemical hybridization assay (FND-EHA) was applied to the detection of two mutations in human lipoprotein lipase (LPL) gene, G188E (one base transition) and Arita (one base deletion). A probe oligodeoxyribonucleotide of 13 bases representing the wild type (WT) sequence of LPL was immobilized on a gold electrode, followed by hybridization with a sample PCR product...
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