Article
Absence of periplasmic DsbA oxidoreductase facilitates export of cysteine-containing passenger proteins to the Escherichia coli cell surface via the Iga beta autotransporter pathway.
Gene - 31 Oct 1996
Jose J, Krämer J, Klauser T, Pohlner J, Meyer T F
Abstract excerpt
The Iga beta autotransporter function of IgA1 protease from Neisseria gonorrhoeae was assessed in Escherichia coli using the Vibrio cholerae toxin B subunit (CtxB) as a heterologous passenger. N-terminal fusions with Iga beta of native CtxB or mutant CtxB protein containing no cysteines were cons...
Topics
- Biological Transport
- Cell Membrane
- Cholera Toxin
- Cysteine
- Escherichia coli
- Isomerases
- Molecular Sequence Data
- Mutation
- Oxidoreductases
- Periplasmic Proteins
- Protein Disulfide-Isomerases
- Recombinant Fusion Proteins
- Serine Endopeptidases
- Vibrio cholerae