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Article

Twin-arginine translocation of active human tissue plasminogen activator in Escherichia coli.

Applied and environmental microbiology - 1 Dec 2005

Kim Jae-Young, Fogarty Elizabeth A, Lu Franklin J, Zhu Hui, Wheelock Geoffrey D, Henderson Lee A, DeLisa Matthew P

Abstract excerpt

When eukaryotic proteins with multiple disulfide bonds are expressed at high levels in Escherichia coli, the efficiency of thiol oxidation and isomerization is typically not sufficient to yield soluble products with native structures. Even when such proteins are secreted into the oxidizing periplasm or expressed in the cytoplasm of cells carrying mutations in the major intracellular disulfide bond reduction...

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Identifiers

PMID: 16332834
DOI: 10.1128/AEM.71.12.8451-8459.2005
PMCID: PMC1317466

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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