Article
Folding quality control in the export of proteins by the bacterial twin-arginine translocation pathway.
Proceedings of the National Academy of Sciences of the United States of America - 13 May 2003
DeLisa Matthew P, Tullman Danielle, Georgiou George
Abstract excerpt
To examine the relationship between folding and export competence by the twin-arginine translocation (Tat) pathway we analyzed the subcellular localization of fusions between a set of eight putative Tat leader peptides and alkaline phosphatase in isogenic Escherichia coli strains that either allow or disfavor the formation of protein disulfide bonds in the cytoplasm. We show that export by the Tat translocator is...
Topics
- Alkaline Phosphatase
- Amino Acid Sequence
- Cytoplasm
- Escherichia coli
- Escherichia coli Proteins
- Kinetics
- Membrane Transport Proteins
- Molecular Sequence Data
- Oxidation-Reduction
- Phenotype
- Protein Folding
- Protein Transport