Article
Exploitation of a chromosomally integrated lactose operon for controlled gene expression in Lactococcus lactis.
FEMS microbiology letters - 1 Feb 1996
Payne J, MacCormick C A, Griffin H G, Gasson M J
Abstract excerpt
Lactococcus lactis MG5267 is a plasmid-free strain in which the lactose operon is integrated in the bacterial chromosome. The chromosomal lacG gene which encodes phospho-beta-galactosidase was inactivated by a double cross-over integration event. Unexpectedly, the resultant mutant was shown to re...
Topics
- Bacteriophages
- Base Sequence
- Biotechnology
- Chromosomes, Bacterial
- Culture Media
- DNA Primers
- DNA, Bacterial
- Gene Expression Regulation, Bacterial
- Lac Operon
- Lactococcus lactis
- Lactose
- Listeria monocytogenes
- Molecular Sequence Data
- Mucoproteins
- Mutation
- Phenotype
- Promoter Regions, Genetic
- Recombinant Proteins