Article
Measurement of Internal pH in Helicobacter pylori by Using Green Fluorescent Protein Fluorimetry.
Journal of bacteriology - 15 Jul 2018
Wen Yi, Scott David R, Vagin Olga, Tokhtaeva Elmira, Marcus Elizabeth A, Sachs George
Abstract excerpt
Helicobacter pylori is an organism known to colonize the normal human stomach. Previous studies have shown that the bacterium does this by elevating its periplasmic pH via the hydrolysis of urea. However, the value of the periplasmic pH was calculated indirectly from the proton motive force equation. To measure the periplasmic pH directly in H. pylori, we fused enhanced green fluorescent protein (EGFP) to the...
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