Article
Sequential enrichment and immunocytochemical visualization of human interferon-alpha-producing cells.
Journal of interferon research - 1 Aug 1990
Feldman M, Fitzgerald-Bocarsly P
Abstract excerpt
Human HLA-DR+ peripheral mononuclear cells (PBMC) produce interferon-alpha (IFN-alpha) in response to herpes simplex virus type 1 (HSV-1) or HSV-1-infected fibroblasts (HSV-FS). We have developed a protocol, based partly on a technique known to enrich for dendritic cells, that allows for a greater than 125-fold enrichment of these IFN-alpha-producing cells. Nylon wood nonadherent PBMC (NWNA) were fractionated on...
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