Article
Sequential enrichment and immunocytochemical visualization of human interferon-alpha-producing cells.
Journal of interferon research - 1 Aug 1990
Feldman M, Fitzgerald-Bocarsly P
Abstract excerpt
Human HLA-DR+ peripheral mononuclear cells (PBMC) produce interferon-alpha (IFN-alpha) in response to herpes simplex virus type 1 (HSV-1) or HSV-1-infected fibroblasts (HSV-FS). We have developed a protocol, based partly on a technique known to enrich for dendritic cells, that allows for a greate...
Topics
- Antigens, Surface
- Cell Count
- Cell Separation
- Cells, Cultured
- Centrifugation, Density Gradient
- Flow Cytometry
- Humans
- Immunohistochemistry
- Interferon Type I
- Killer Cells, Natural
- Leukocytes, Mononuclear
- Microscopy, Electron
- Monocytes
- Phenotype
- Simplexvirus
- T-Lymphocytes
- Ultraviolet Rays