Article
Detection of individual mouse splenic T cells producing IFN-gamma and IL-5 using the enzyme-linked immunospot (ELISPOT) assay.
Journal of immunological methods - 27 Mar 1990
Taguchi T, McGhee J R, Coffman R L, Beagley K W, Eldridge J H, Takatsu K, Kiyono H
Abstract excerpt
Although several sensitive and specific assays have been developed to quantify murine cytokines, these assays do not allow individual cells to be correlated with the specific cytokines they produce. The purpose of this study was to develop a sensitive and reproducible method for the detection of individual T cells which secrete either interferon-gamma (IFN-gamma) or interleukin-5 (IL-5). We have used an...
Topics
- Animals
- Antibodies, Monoclonal
- Immunoenzyme Techniques
- Interferon-gamma
- Interleukin-5
- Mice
- Mice, Inbred C3H
- Phenotype
- T-Lymphocytes
- T-Lymphocytes, Helper-Inducer