Article
Directed mutagenesis, Ser-56 to Pro, of Bacillus subtilis phosphatidylserine synthase drastically lowers enzymatic activity and relieves amplification toxicity in Escherichia coli.
Bioscience, biotechnology, and biochemistry - 1 Apr 1996
Saha S K, Furukawa Y, Matsuzaki H, Shibuya I, Matsumoto K
Abstract excerpt
Amino acid residue 56 of the phosphatidylserine synthase of Bacillus subtilis was changed from Ser to Pro by using modified primers in PCR amplification of its structural gene, pssBS. When an Escherichia coli mutant lacking its own phosphatidylserine synthase harbored a plasmid carrying this allele, the Mn(2+)-requiring Bacillus-type synthase activity, as assayed in vitro, was at least six-fold lower than that...
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