Article
Sequence and inactivation of the pss gene of Escherichia coli. Phosphatidylethanolamine may not be essential for cell viability.
The Journal of biological chemistry - 15 Mar 1991
DeChavigny A, Heacock P N, Dowhan W
Abstract excerpt
Phosphatidylethanolamine is the only zwitterionic phospholipid in Escherichia coli and accounts for 70-80% of the total glycerophospholipids of this organism. To investigate the function of phosphatidylethanolamine in E. coli, we constructed an inactivated allele (pss93::kan) of the gene encoding the phosphatidylserine synthase which catalyzes the committed step to the synthesis of phosphatidylethanolamine....
Topics
- Amino Acid Sequence
- Base Sequence
- Blotting, Southern
- Cations, Divalent
- Cell Survival
- Chromatography, Thin Layer
- DNA, Bacterial
- Escherichia coli
- Fatty Acids
- Genes, Bacterial
- Molecular Sequence Data