Article
Mutational analysis of the nucleoid-associated protein HBsu of Bacillus subtilis.
Molecular & general genetics : MGG - 1 Dec 1998
Köhler P, Marahiel M A
Abstract excerpt
The essential nucleoid-associated protein HBsu of Bacillus subtilis comprises 92 residues, 20% of which are basic amino acids. To investigate the role of the residues located within the DNA-binding arm, the arginine residues R58 and R61 were changed to leucine, while lysine residues K80 and K86 were replaced by alanine. All altered proteins exhibited a reduction in DNA binding capacity, ranging from 10% to 30% of...
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