Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Procyclic Trypanosoma brucei cell lines deficient in ornithine decarboxylase activity.

Molecular and biochemical parasitology - 1 Jun 1996

Li F, Hua S B, Wang C C, Gottesdiener K M

Abstract excerpt

Ornithine decarboxylase (ODC) is a rate limiting enzyme in the biosynthesis of polyamines. We report here the construction of ODC gene deficient Trypanosoma brucei brucei cell lines by homologous recombination and disruption of the two alleles of the ODC gene. With our first stable transfection vector, we replaced the 2.8 kb SacII ODC gene-containing fragment with a hygromycin-B-phosphotransferase gene (hph)...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Alleles
Share to Cell Cycle
Share to Gene Deletion

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Gene Expression

Share to Mutagenesis, Insertional

Share to Recombination, Genetic

Share to RNA, Messenger

Share to Transfection

Source

Identifiers

PMID: 8813692
DOI: 10.1016/s0166-6851(96)02630-8

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Procyclic Trypanosoma brucei cell lines deficient in ornithine decarboxylase activity.PMID 8813692Read ↗

Trypanosoma brucei brucei: characterization of an ODC null bloodstream form mutant and the action of alpha-difluoromethylornithine.Semantic proximity Read ↗

A Trypanosoma brucei bloodstream form mutant deficient in ornithine decarboxylase can protect against wild-type infection in mice.Semantic proximity Read ↗

Ornithine decarboxylase gene deletion mutants of Leishmania donovani.Semantic proximity Read ↗

S-adenosylmethionine decarboxylase from Leishmania donovani. Molecular, genetic, and biochemical characterization of null mutants and overproducers.Semantic proximity Read ↗

iCODA: RNAi-based inducible knock-in system in Trypanosoma brucei.Semantic proximity Read ↗

pRIBOTEX expression vector: a pTEX derivative for a rapid selection of Trypanosoma cruzi transfectantsSemantic proximity Read ↗

Cloning and disruption of the ornithine decarboxylase gene of Ustilago maydis: evidence for a role of polyamines in its dimorphic transition.Semantic proximity Read ↗

Polyamine homoeostasis as a drug target in pathogenic protozoa: peculiarities and possibilitiesSemantic proximity Read ↗

Spermidine synthase is required for virulence of Leishmania donovani.Semantic proximity Read ↗

A new model for disruption of the ornithine decarboxylase gene, SPE1, in Saccharomyces cerevisiae exhibits growth arrest and genetic instability at the MAT locus.Semantic proximity Read ↗

Phenotypic analysis of trypanothione synthetase knockdown in the African trypanosome.Semantic proximity Read ↗

Genetic manipulation indicates that ARD1 is an essential Nα-acetyltransferase in Trypanosoma bruceiSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.