Article
Procyclic Trypanosoma brucei cell lines deficient in ornithine decarboxylase activity.
Molecular and biochemical parasitology - 1 Jun 1996
Li F, Hua S B, Wang C C, Gottesdiener K M
Abstract excerpt
Ornithine decarboxylase (ODC) is a rate limiting enzyme in the biosynthesis of polyamines. We report here the construction of ODC gene deficient Trypanosoma brucei brucei cell lines by homologous recombination and disruption of the two alleles of the ODC gene. With our first stable transfection v...
Topics
- Alleles
- Animals
- Cell Cycle
- Culture Media
- Gene Deletion
- Gene Expression
- Genes, Protozoan
- Mutagenesis, Insertional
- Ornithine Decarboxylase
- Polyamines
- Putrescine
- RNA, Messenger
- RNA, Protozoan
- Recombination, Genetic
- Transfection
- Trypanosoma brucei brucei