Article
A new model for disruption of the ornithine decarboxylase gene, SPE1, in Saccharomyces cerevisiae exhibits growth arrest and genetic instability at the MAT locus.
The Biochemical journal - 15 Nov 1995
Schwartz B, Hittelman A, Daneshvar L, Basu H S, Marton L J, Feuerstein B G
Abstract excerpt
Ornithine decarboxylase (ODC) is a rate-determining enzyme of the polyamine-biosynthetic pathway. We sought to produce cells with impaired ODC function in order to study the biological functions of polyamines. Saccharomyces cerevisiae strains were obtained by one-step gene replacement of a 900 bp...
Topics
- Blotting, Southern
- Cell Cycle
- Cell Division
- Culture Media
- Flow Cytometry
- Genes, Fungal
- Genes, Mating Type, Fungal
- Genetic Markers
- Genotype
- Heterozygote
- Mating Factor
- Mutagenesis
- Ornithine Decarboxylase
- Peptides
- Ploidies