Article
Cloning and disruption of the ornithine decarboxylase gene of Ustilago maydis: evidence for a role of polyamines in its dimorphic transition.
Microbiology (Reading, England) - 1 Jul 1997
Guevara-Olvera Lorenzo, Xoconostle-Cázares Beatriz, Ruiz-Herrera José
Abstract excerpt
The gene encoding ornithine decarboxylase (ODC) from Ustilago maydis was cloned. A conserved PCR product amplified from U. maydis DNA was synthesized and used to screen a genomic library of the fungus. Alignment of its deduced protein sequence with those of other cloned ODCs showed a high degree of homology. Gene replacement was obtained by removal of a central part of the gene and insertion of the hygromycin...
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