Article
A supercoil-specific endonuclease from Salmonella typhimurium cleaves both negatively and positively supercoiled DNA.
Biochemistry and molecular biology international - 1 May 1996
Zargar M A, Chakravorty M
Abstract excerpt
An enzyme which specifically cleaves supercoiled DNA to linear form through nicked circular form as intermediate was isolated from rifampicin-resistant mutant of Salmonella typhimurium, rif 39. The enzyme activity was stimulated by Mg+2, whereas Ca+2 had no effect. It does not require ATP for its activity. No activity could be detected with relaxed or single stranded circular DNAs. The molecular weight of the...
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