Article
Two-step cloning and expression in Escherichia coli of the DNA restriction-modification system StyLTI of Salmonella typhimurium.
Journal of bacteriology - 1 Feb 1991
De Backer O, Colson C
Abstract excerpt
The StyLTI restriction-modification system is common to most strains of the genus Salmonella, including Salmonella typhimurium. We report here the two-step cloning of the genes controlling the StyLTI system. The StyLTI methylase gene (mod) was cloned first. Then, the companion endonuclease gene (...
Topics
- Cloning, Molecular
- DNA Modification Methylases
- DNA Restriction Enzymes
- Deoxyribonucleases, Type III Site-Specific
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Genes, Bacterial
- Phenotype
- Plasmids
- Restriction Mapping
- Salmonella typhimurium
- Site-Specific DNA-Methyltransferase (Adenine-Specific)
- Transformation, Bacterial