Article
Free energy perturbation calculations on binding and catalysis after mutating Asn 155 in subtilisin.
Nature - 1 Jan 2000
Rao S N, Singh U C, Bash P A, Kollman P A
Abstract excerpt
Site-directed mutagenesis is a very powerful approach to altering the biological functions of proteins, the structural stability of proteins and the interactions of proteins with other molecules. Several experimental studies in recent years have been directed at estimating the changes in catalytic properties, (rates of binding and catalysis) in site-directed mutants of enzymes compared to the native enzymes....
Topics
- Asparagine
- Catalysis
- Computer Simulation
- Kinetics
- Models, Molecular
- Mutation
- Protein Conformation
- Structure-Activity Relationship
- Subtilisins
- Thermodynamics