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Can the stability of protein mutants be predicted by free energy calculations?

Protein engineering - 1 Apr 1993

Shi Y Y, Mark A E, Wang C X, Huang F, Berendsen H J, van Gunsteren W F

Abstract excerpt

The use of free energy simulation techniques in the study of protein stability is critically evaluated. Results from two simulations of the thermostability mutation Asn218 to Ser218 in Subtilisin are presented. It is shown that components of the free energy change can be highly sensitive to the computational details of the simulation leading to the conclusion that free energy calculations cannot currently be used...

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PMID: 8506263
DOI: 10.1093/protein/6.3.289

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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