Article
Immunoreactive core peptides of hepatitis C virus produced in Escherichia coli and in vitro DNA amplification-restricted transcription-translation system.
Journal of virological methods - 1 May 1996
Esumi M, Hayashi N, Takahashi H, Shikata T, Moriyama M, Arakawa Y, Eto T, Nishihara T, Nozaki C, Mizuno K
Abstract excerpt
Three kinds of hepatitis C virus (HCV) core peptides were produced directly and efficiently in E. coli: 1-120 aa of the C region as NCC, 1-157 aa as NCCT and 1-190 aa as NCCL. These peptides were estimated to be 16, 22 and 24 kDa, respectively, by SDS-polyacrylamide gel electrophoresis. The processing to produce p22 core protein observed in insect cells and mammalian systems did not occur in E. coli. These...
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