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[Prokaryotic expression and characterization of truncated mutant capsid protein of genotype IV hepatitis E virus].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology - 1 Mar 2014

Qiu Yilan, Wu Junwen, Qiu Guo, Li Sang, Li Ye, Liu Shengzi, Liu Rushi

Abstract excerpt

A truncated mutant of the Open Reading Frame 2 (ORF2, aa384-606) was amplified from cDNA of genotype IV hepatitis E virus (HEV) by polymerase chain reaction (PCR), subcloned to expression plasmid pTO-T7, and expressed in Escherichia coli. SDS-PAGE and Western blotting were used to detect and identify the recombinant protein, namely rP24. After washing of inclusion bodies, dissolving in denaturing agents,...

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PMID: 25007574

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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