Article
[Prokaryotic expression and characterization of truncated mutant capsid protein of genotype IV hepatitis E virus].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology - 1 Mar 2014
Qiu Yilan, Wu Junwen, Qiu Guo, Li Sang, Li Ye, Liu Shengzi, Liu Rushi
Abstract excerpt
A truncated mutant of the Open Reading Frame 2 (ORF2, aa384-606) was amplified from cDNA of genotype IV hepatitis E virus (HEV) by polymerase chain reaction (PCR), subcloned to expression plasmid pTO-T7, and expressed in Escherichia coli. SDS-PAGE and Western blotting were used to detect and iden...
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