Article
Protease-like sequence in hepatitis B virus core antigen is not involved in the cleavage processes of core protein in Escherichia coli.
Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology - 1 Feb 1991
Hwang L H, Lin Y J, Lai W C, Lo M S
Abstract excerpt
A DNA fragment, coding for hepatitis core antigen (HBcAg), was amplified by polymerase chain reaction and inserted into a lambda PL promoter-derived expression vector. The recombinant plasmid was transformed into Escherichia coli and proteins produced after heat induction were analyzed. In addition to the 21 kDa HBcAg protein, several smaller related polypeptides, particularly one of 17 kDa in size, were also...
Topics
- Aspartic Acid Endopeptidases
- Base Sequence
- Chromosome Deletion
- Escherichia coli
- Genetic Vectors
- Hepatitis B Core Antigens
- Molecular Sequence Data
- Molecular Weight
- Mutation
- Plasmids
