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Article

Overexpression, purification, and generation of a thermostable variant of Zymomonas mobilis fructokinase.

Protein expression and purification - 1 Jun 1996

King K, Phan P, Rellos P, Scopes R K

Abstract excerpt

The gene encoding fructokinase (EC 2.7.1.4) from Zymomonas mobilis has been expressed at high level in Escherichia coli by modifying the ribosome binding site using the polymerase chain reaction. A simple two-step purification from extracts of the recombinant cells results in highly purified enzyme suitable for use in fructose determination. Using the polymerase chain reaction in mutagenic conditions, a variant...

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PMID: 8776754
DOI: 10.1006/prep.1996.0055

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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