Article
Overexpression, purification, and generation of a thermostable variant of Zymomonas mobilis fructokinase.
Protein expression and purification - 1 Jun 1996
King K, Phan P, Rellos P, Scopes R K
Abstract excerpt
The gene encoding fructokinase (EC 2.7.1.4) from Zymomonas mobilis has been expressed at high level in Escherichia coli by modifying the ribosome binding site using the polymerase chain reaction. A simple two-step purification from extracts of the recombinant cells results in highly purified enzy...
Topics
- DNA Primers
- Enzyme Stability
- Escherichia coli
- Fructokinases
- Gene Expression Regulation, Bacterial
- Glucose
- Half-Life
- Hot Temperature
- Mutation
- Plasmids
- Polymerase Chain Reaction
- Recombinant Proteins
- Ribosomes
- Sequence Analysis, DNA
- Zymomonas