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Crystallization and preliminary crystallographic analysis of a thermostable mutant of kanamycin nucleotidyltransferase.

Archives of biochemistry and biophysics - 15 May 1992

Kanikula A M, Liao H H, Sakon J, Holden H M, Rayment I

Abstract excerpt

A thermostable mutant of kanamycin nucleotidyltransferase isolated by cloning and selection for kanamycin resistance in Bacillus stearothermophilus at 70 degrees C has been crystallized in a form suitable for high-resolution diffraction analysis. This enzyme catalyzes nucleotidyl group transfer from nucleoside triphosphates such as ATP to hydroxyl groups of various aminoglycosides, thus inactivating the...

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PMID: 1315501
DOI: 10.1016/0003-9861(92)90479-g

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