Article
Bacillus subtilis DNA polymerase III: complete sequence, overexpression, and characterization of the polC gene.
Gene - 1 Feb 1991
Hammond R A, Barnes M H, Mack S L, Mitchener J A, Brown N C
Abstract excerpt
Genomic DNA encompassing polC, the structural gene specifying Bacillus subtilis DNA polymerase III (PolIII), was sequenced and found to contain a 4311-bp open reading frame (ORF) encoding a 162.4-kDa polypeptide of 1437 amino acids (aa). The ORF was engineered into an Escherichia coli expression plasmid under the control of the coliphage lambda repressor. Derepression of E. coli transformants carrying the...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
