Bacillus subtilis DNA polymerase III: complete sequence, overexpression, and characterization of the polC gene.

doi:10.1016/0378-1119(91)90100-p

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Article

Bacillus subtilis DNA polymerase III: complete sequence, overexpression, and characterization of the polC gene.

Gene - 1 Feb 1991

Hammond R A, Barnes M H, Mack S L, Mitchener J A, Brown N C

Abstract excerpt

Genomic DNA encompassing polC, the structural gene specifying Bacillus subtilis DNA polymerase III (PolIII), was sequenced and found to contain a 4311-bp open reading frame (ORF) encoding a 162.4-kDa polypeptide of 1437 amino acids (aa). The ORF was engineered into an Escherichia coli expression plasmid under the control of the coliphage lambda repressor. Derepression of E. coli transformants carrying the...

Topics

Alleles
Amino Acid Sequence
Bacillus subtilis
Base Sequence
Cloning, Molecular
Codon
DNA Polymerase III
DNA, Bacterial
DNA-Directed DNA Polymerase
Escherichia coli
Gene Expression

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Genes, Bacterial

Molecular Sequence Data

Open Reading Frames

Plasmids

Protein Biosynthesis

Sequence Homology, Nucleic Acid

Transformation, Bacterial

Source

Open in PubMed

Identifiers

PMID: 1901559
DOI: 10.1016/0378-1119(91)90100-p

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1901559-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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