Localization of the exonuclease and polymerase domains of Bacillus subtilis DNA polymerase III.

doi:10.1016/0378-1119(92)90601-k

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Article

Localization of the exonuclease and polymerase domains of Bacillus subtilis DNA polymerase III.

Gene - 1 Feb 1992

Barnes M H, Hammond R A, Kennedy C C, Mack S L, Brown N C

Abstract excerpt

Structural gene mutants were cloned and exploited to identify the major catalytic domains of Bacillus subtilis DNA polymerase III (BsPolIII), a 162.4-kDa [1437 amino acids (aa)] polymerase: 3'-5' exonuclease (Exo) required for replicative DNA synthesis. Analysis of the sequence, mutagenicity, and...

Topics

Amino Acid Sequence
Bacillus subtilis
Base Sequence
Cloning, Molecular
DNA Polymerase III
DNA, Bacterial
Drug Resistance, Microbial
Exonucleases
Molecular Sequence Data
Mutation
Nucleotides
Phenotype
Restriction Mapping
Sequence Alignment
Uracil

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