Article
Quantitative assessment of the human TCRBV repertoire by competitive PCR.
Journal of immunological methods - 14 Aug 1996
Uhrberg M, Wernet P
Abstract excerpt
A novel quantitative protocol was developed for the measurement of the relative expression levels of the human TCRBV genes based on reverse transcription (RT) and subsequent competitive polymerase chain reaction (cPCR). Competitor DNA templates for the analysis of 24 different TCRBV families were generated by a simple and rapid one-step PCR procedure with a special PCR primer, which introduces a deletion in the...
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