Article
Using differential scanning calorimetry to elucidate metal-protein binding sites in alpha- and gamma-chymotrypsin.
Bioseparation - 1 Oct 1995
Sagar S L, Domach M M
Abstract excerpt
Crystalline alpha-chymotrypsin preparations are contaminated by the post translational variant, gamma-chymotrypsin. The contaminant can account for 5-50 weight percent of the preparation based on thermal analysis. Such contamination can be problematic because this serine protease has both commercial and deactivation model system utility, and the presence of the contaminant may not be detectable by activity...
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