Article
Subunit association of gamma-glutamyltranspeptidase of Escherichia coli K-12.
Journal of biochemistry - 1 Dec 1995
Hashimoto W, Suzuki H, Nohara S, Tachi H, Yamamoto K, Kumagai H
Abstract excerpt
gamma-Glutamyltranspeptidase [EC 2.3.2.2] of Escherichia coli K-12 consists of one large subunit and one small subunit, which can be separated from each other by high-performance liquid chromatography. Using ion spray mass spectrometry, the masses of the large and the small subunit were determine...
Topics
- Amino Acid Sequence
- Base Sequence
- Blotting, Western
- Chromatography, High Pressure Liquid
- Cloning, Molecular
- DNA Primers
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Genotype
- Macromolecular Substances
- Mass Spectrometry
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Plasmids
- Polymerase Chain Reaction
- Recombinant Proteins
- Restriction Mapping
- gamma-Glutamyltransferase