Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Enhanced catalytic efficiency of aminoglycoside phosphotransferase (3')-IIa achieved through protein fragmentation and reassembly.

Journal of molecular biology - 14 Oct 2005

Paschon David E, Patel Zarana S, Ostermeier Marc

Abstract excerpt

Many monomeric proteins can be split into two fragments, yet the two fragments can associate to make an active heterodimer. However, for most locations in a protein such a conversion is not feasible, presumably due to inefficient assembly or improper folding of the fragments. For some locations, this can be overcome by fusion of the fragments to dimerization domains that facilitate correct assembly. A variety of...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Catalysis
Share to Dimerization
Share to Drug Resistance

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Kanamycin

Share to Kanamycin Kinase

Share to Peptide Fragments

Share to Peptide Library

Share to Protein Engineering

Source

Identifiers

PMID: 16168439
DOI: 10.1016/j.jmb.2005.08.026

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Enhanced catalytic efficiency of aminoglycoside phosphotransferase (3')-IIa achieved through protein fragmentation and reassembly.PMID 16168439Read ↗

Purification and characterization of aminoglycoside 3'-phosphotransferase type IIa and kinetic comparison with a new mutant enzyme.Semantic proximity Read ↗

Site-specific mutations of conserved C-terminal residues in aminoglycoside 3'-phosphotransferase II: phenotypic and structural analysis of mutant enzymes.Semantic proximity Read ↗

Molecular and genetic approach to enhancing protein secretion.Semantic proximity Read ↗

Error-prone PCR mutagenesis reveals functional domains of a bacterial transcriptional activator, TraJ.Semantic proximity Read ↗

Irreversible misfolding of diacylglycerol kinase is independent of aggregation and occurs prior to trimerization and membrane association.Semantic proximity Read ↗

The glucose transporter of the Escherichia coli phosphotransferase system: linker insertion mutants and split variants.Semantic proximity Read ↗

Autoinhibitory regulation of TrwK, an essential VirB4 ATPase in type IV secretion systems.Semantic proximity Read ↗

Mechanism of phosphoryl transfer in the dimeric IIABMan subunit of the Escherichia coli mannose transporter.Semantic proximity Read ↗

A mutant neomycin phosphotransferase II gene reduces the resistance of transformants to antibiotic selection pressure.Semantic proximity Read ↗

Systematic analysis of a conserved region of the aminoglycoside 6'-N-acetyltransferase type Ib.Semantic proximity Read ↗

ATPase-defective derivatives of Escherichia coli DnaK that behave differently with respect to ATP-induced conformational change and peptide release.Semantic proximity Read ↗

Characterization of the active site of S. aureus monofunctional glycosyltransferase (Mtg) by site-directed mutation and structural analysis of the protein complexed with moenomycin.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.