Article
Escherichia coli gamma-glutamyltranspeptidase mutants deficient in processing to subunits.
Biochemical and biophysical research communications - 30 Nov 1992
Hashimoto W, Suzuki H, Nohara S, Kumagai H
Abstract excerpt
Arginyl residues 513 and 571 of Escherichia coli K-12 gamma-glutamyl-transpeptidase (EC 2.3.2.2) were substituted with alanyl and glycyl residues, respectively, by oligonucleotide-directed in vitro mutagenesis. Both mutants were devoid of the enzymatic activity. On Western blot analysis, we found that both mutants accumulated a gamma-glutamyltranspeptidase precursor which was not processed into large and small...
Topics
- Alanine
- Amino Acid Sequence
- Arginine
- Base Sequence
- Blotting, Western
- Escherichia coli
- Genotype
- Glycine
- Kinetics
- Macromolecular Substances
- Molecular Sequence Data