Article
Complementation of a Toxoplasma gondii ROP1 knock-out mutant using phleomycin selection.
Molecular and biochemical parasitology - 1 Oct 1995
Soldati D, Kim K, Kampmeier J, Dubremetz J F, Boothroyd J C
Abstract excerpt
The ROP1 gene of Toxoplasma gondii encodes a rhoptry protein that has been implicated in host cell invasion by this obligate intracellular protozoan. To further explore the function of this protein, we created a ROP1 deletion mutant by transfection with a plasmid encoding the bacterial chloramphenicol acetyltransferase (cat) gene flanked by ROP1 genomic sequences. Selection for chloramphenicol resistance yielded...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
