Identification of temperature-sensitive mutants of the human immunodeficiency virus type 1 protease through saturation mutagenesis. Amino acid side chain requirements for temperature sensitivity.

Abstract excerpt

Human immunodeficiency virus type 1 encodes a protease whose activity is required for the production of infectious virus. An Escherichia coli expression and processing assay system was used to screen 285 protease mutants for temperature-sensitive activity. Fourteen protease mutants had a temperat...

Topics

• Amino Acids
• Enzyme Stability
• HIV Protease
• Models, Molecular
• Mutagenesis
• Phenotype
• Protein Conformation
• Temperature