Modifying Human Thymidylate Kinase to Potentiate Azidothymidine Activation

doi:10.1074/jbc.274.50.35289

Article

Modifying Human Thymidylate Kinase to Potentiate Azidothymidine Activation

1 Dec 1999

Abstract excerpt

Based on the knowledge of the crystal structures of yeast and Escherichia coli thymidylate kinases (TmpKs) and the observation that TmpK from E. coli can phosphorylate azidothymidine monophosphate (AZT-MP) much more efficiently than either the yeast or the highly homologous human enzyme, we have engineered yeast and human TmpKs to obtain enzymes that have dramatically improved AZT-MP phosphorylation properties....

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