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Article

A simple Escherichia coli system for monitoring HIV protease activity: analysis of two temperature-sensitive protease mutants.

AIDS research and human retroviruses - 1 Apr 1990

Rockenbach S K, Olsen M K, Tomich C S

Abstract excerpt

A simple Escherichia coli system has been developed for the detection of human immunodeficiency virus (HIV) protease activity. In this system, the protease sequence is placed downstream of the HIV gag polypeptide in an operon arrangement. Upon expression of the operon, gag serves as the substrate for the protease; the level of protease activity can be determined by measurement of the cleavage product of gag in...

Topics

Endopeptidases
Escherichia coli
Gene Products, gag
Gene Products, pol
Genetic Vectors
HIV Protease
Mutation
Operon
Temperature

Source

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 2187504
DOI: 10.1089/aid.1990.6.543

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2187504-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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