Article
Screening for human mitochondrial DNA polymorphisms with denaturing gradient gel electrophoresis.
Human genetics - 1 Aug 1996
Hanekamp J S, Thilly W G, Chaudhry M A
Abstract excerpt
A method involving denaturing gradient gel electrophoresis (DGGE) was developed to detect mitochondrial DNA (mtDNA) polymorphisms in human peripheral T-lymphocytes. DGGE analysis of 100- to 200-bp sequences of low melting temperature domains within the origin/membrane attachment site, NADH dehydrogenase subunit I, cytochrome c oxidase subunit I and two overlapping regions of the tRNA glycine/NADH dehydrogenase...
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