Screening for human mitochondrial DNA polymorphisms with denaturing gradient gel electrophoresis.

doi:10.1007/s004390050201

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Article

Screening for human mitochondrial DNA polymorphisms with denaturing gradient gel electrophoresis.

Human genetics - 1 Aug 1996

Hanekamp J S, Thilly W G, Chaudhry M A

Abstract excerpt

A method involving denaturing gradient gel electrophoresis (DGGE) was developed to detect mitochondrial DNA (mtDNA) polymorphisms in human peripheral T-lymphocytes. DGGE analysis of 100- to 200-bp sequences of low melting temperature domains within the origin/membrane attachment site, NADH dehydr...

Topics

Base Sequence
DNA Primers
DNA, Mitochondrial
Electrophoresis, Polyacrylamide Gel
Genetic Testing
Genetic Variation
Humans
Molecular Sequence Data
Polymerase Chain Reaction
Polymorphism, Genetic
T-Lymphocytes

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