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Article

Denaturing gradient gel method for mapping single base changes in human mitochondrial DNA.

Analytical biochemistry - 1 Aug 1991

Yoon K L, Modica-Napolitano J S, Ernst S G, Aprille J R

Abstract excerpt

A denaturing gradient gel electrophoresis (DGGE) method is described that detects even single base pair changes in mitochondrial DNA (mtDNA). In this method, restriction fragments of mtDNA are electrophoresed in a urea/formamide gradient gel at 60 degrees C. Migration distance of each mtDNA fragment in the gel depends on melting behavior which reflects base composition. Fragments are located by Southern blotting...

Topics

Base Sequence
DNA, Mitochondrial
Electrophoresis
Humans
Mitochondria, Liver
Mitochondria, Muscle
Molecular Sequence Data
Mutation
Nucleic Acid Denaturation
Polymorphism, Genetic
Polymorphism, Restriction Fragment Length

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 1685630
DOI: 10.1016/0003-2697(91)90489-g

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1685630-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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